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Exosome Sequencing (CAT#: STEM-MB-0075-WXH)

Introduction

Exosomes are tiny membrane vesicles that can be secreted by most cells and are distributed in body fluids such as peripheral blood, urine, saliva, ascites, and amniotic fluid. It comes from various sources and can come from various types of cells, and its content includes proteins, lipids and nucleic acids, etc., especially the non-coding RNA (miRNA, lncRNA, circRNA, etc.) found in it makes it more meaningful. Sequencing exosomal RNA through high-throughput technology can quickly and efficiently obtain comprehensive information on exosomal RNA, which is an ideal means for disease diagnosis and follow-up. At present, the research on RNA based on exosomes mainly focuses on miRNA, lncRNA, circRNA, mRNA and other types.




Applications

• Study of lncRNAs as potential therapeutic targets to treat drug resistance;
• Study as a molecular marker for disease diagnosis, monitoring and prognosis;
• lncRNAs are rich in miRNA seed sequences, which can be used to study its regulatory mechanism in the process of carcinogenesis, etc.;
• Study exosome-mediated ncRNA transport regulates cell function;
• Research on the mechanism of tumorigenesis and development mediated by exosomal ncRNA;
• The interaction between miRNA and circRNA, and its regulatory role in the occurrence and development of tumors and other diseases.

Procedure

1. RNA extraction and quality inspection
2. Library construction
3. Sequencing
4. Standardized Analysis

Notes

Customers provide exosomes or exosomes isolated by our company

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