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Exosomes are tiny membrane vesicles that can be secreted by most cells and are distributed in body fluids such as peripheral blood, urine, saliva, ascites, and amniotic fluid. It comes from various sources and can come from various types of cells, and its content includes proteins, lipids and nucleic acids, etc., especially the non-coding RNA (miRNA, lncRNA, circRNA, etc.) found in it makes it more meaningful. Sequencing exosomal RNA through high-throughput technology can quickly and efficiently obtain comprehensive information on exosomal RNA, which is an ideal means for disease diagnosis and follow-up. At present, the research on RNA based on exosomes mainly focuses on miRNA, lncRNA, circRNA, mRNA and other types.