Fast cycling PCR (CAT#: STEM-MB-0203-WXH)

Introduction

Fast cycling PCR is a PCR-based technology that allows amplification of specific PCR products with significantly reduced cycling time.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

The principle in this process is the same as conventional PCR, the only difference being the time of amplification.
The buffer used in this PCR increases the affinity of Taq DNA polymerases for short single-stranded DNA fragments, reducing the time required for successful primer annealing to just 5 seconds.

Applications

Fast cycling PCR is essential for processes requiring quick cycles and also helps in the rapid diagnosis of diseases and mutations.

Procedure

General procedure:
1. Denaturation
2. Annealing
3. Extension

Other recommended products

Analysis of Sarcocystis Spp. by Real-Time PCR Method (CAT#: STEM-MB-3257-LGZ)
Analysis of Microsporum Nanum by Real-Time PCR Method (CAT#: STEM-MB-3264-LGZ)
Analysis of Edwardsiella Spp. by Real-Time PCR Method (CAT#: STEM-MB-3024-LGZ)
Analysis of Nematodirus Filicollis by Real-Time PCR Method (CAT#: STEM-MB-4048-LGZ)
Analysis of LINC01594 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1462-LGZ)
Analysis of Measles Virus by Real-Time PCR Method (CAT#: STEM-MB-3160-LGZ)
Analysis of Chlamydophila Spp. by Real-Time PCR Method (CAT#: STEM-MB-2661-LGZ)
Analysis of Clostridium Botulinum TypeA by Real-Time PCR Method (CAT#: STEM-MB-2648-LGZ)