The spin columns are designed for protein purification using up to 700 μl of desalting or affinity resins. The total volume of the column was 1.1 ml, so 400 μl of reservoir was available for the q wash and elution steps. The column features a screw cap that is polypropylene with a snap off end above a 30 micron hydrophilic polyethylene frit. Columns are available in 1, 5 and 2ml centrifuge tubes as collection tubes. The frit is mounted on a column. Simply add the affinity resin and sample to a spin column, then use a microcentrifuge to efficiently wash away contaminants and elute the purified sample without losing any resin in the process. Spin columns allow you to affinity purify more proteins in less time.
Specification
Resin Volume: 20-700µl Total Volume: 1.1ml Reservoir Volume: 400µl Closure: Snap off cap Cap: Screw Cap with O-ring Frit: 30µm hydrophilic polyethylene