G-Sep™ Butyl Agarose 6 Fast Flow, 200ml, 786-958, G-BIOSCIENCES (CAT#: STEM-C-3281-LGZ)

G-Sep™ Butyl Sepharose Fast Flow (FF) is a 6% agarose cross-linked with butyl for hydrophobic interaction chromatography (HIC). This resin is used to separate biomolecules on the basis of relative hydrophobicity.

G-Sep™ Agarose for HIC can also immobilize octyl and phenyl groups on 6% cross-linked agarose beads via neutral and highly stable ether linkages.

Size: 200ml
Matrix: Highly cross-linked Agarose, 6%
Bead form: Spherical, diameter 50-160μm
Ligand: Butyl
Ligand Concentration: About 40μmol /ml
Binding Capacity: About 20mg HSA/ml resin
pH stability Working Range: 3-13
pH stability Cleaning-in-Place (CIP): 2-14
Maximum Pressure (MPa): 0.3
Maximum Flow Velocity: 450cm/h
Exclusion Limit (Globular Proteins): 4 x 106
Physical Stability: Negligible volume variation due to changes in pH or ionic strength
Chemical Stability: Stable to all commonly used aqueous buffers: 1 M NaOH, 8 M urea, 8 M guanidine hydrochloride, 70% ethanol
Sterilization: Autoclavable, In 1M NaOH,121 °C, pH 7, for 30 min
Storage Conditions: 4 to 30°C, 20% Ethanol

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