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G-Sep™ Dynamic Size Exclusion Column (16 mm ID; 60 cm length, 3-70 kDa HR resin), Mean Partice Size 35 μm, 786-1299, G-BIOSCIENCES (CAT#: STEM-C-3278-LGZ)

Cat Number: STEM-C-3278-LGZ

Application: G-Sep™ Size Exclusion Columns are high resolution preparative grade columns with separation range of 6-600 kDa for G-Sep™ Size Exclusion Column, 6-600 HR resin and 3-70 kDa for G-Sep™ Size Exclusion Column, 6-600 HR resin. <br />G-Sep™ Size Exclusion Columns are used in sepration of biomolecules based on size (size exclusion chromatography)

Model: 786-1299

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Description

Size exclusion chromatography or gel filtration is a widely used technique for separating proteins and other molecules by size.

G-Sep™ Size Exclusion Columns are composed of a high performance rigid resin composed of agarose and dextran copolymer. Columns are available in 11/30, 16/60, and 26/60 column formats and have two separation ranges, 3 kDa to 70 kDa and 6 kDa to 600 kDa.

G-Sep™ Size Exclusion Columns, 3-70 kDa with performance similar to Superdex™ 75 pg Columns, G-Sep™ Size Exclusion Columns, 6-600 kDa with performance similar to Superdex™ 200 pg Columns. G-Sep™ Size Exclusion Columns are available in two formats, the classic non-adjustable end adapter and the dynamic adjustable end adapter. G-Sep™ dynamic size exclusion column format is 16/60.

Highlights:

High-resolution preparative grade columns with a separation range of 6-600 kDa (G-Sep™ Size Exclusion Column, 6-600 HR Resin) and 3-70 kDa (G-Sep™ Size Exclusion Column, 6-600 HR Resin).

The column is certified for height equivalent to theoretical plate (HETP) and peak asymmetry (number of theoretical plates: >10,000 N/m and Peak Asymmetry: 0.7-1.5).

G-Sep™ Size Exclusion Columns are available in two formats; the classic one with non-adjustable end adapters and the dynamic one with adjustable end adapters

G-Sep™ size exclusion columns are formatted as 11/30, 16/60 and 26/60. G-Sep™ dynamic size exclusion column format is 16/60.

Specification

Size: 1 column
Matrix: Highly cross-linked polysaccharide composite of dextran and agarose.
Mean Partice Size: 35 μm
Column body maximum pressure: 6Bar, 0.6 MPa (3-70 HR columns); 5Bar, 0.5 MPa (6-600 HR columns)
Maximun resin pressure: 4 Bar (3-70 HR columns); 3 Bar (6-600 HR columns)
Operatin Flow velocity: 10-50 cm/hr
pH stability: 2-14 (short term), 3-12 (long term)
Working temperature: +4°C to +30°C
Chemical stability: Stable with coomonly used aqueous buffers, 1 M cetic acid, 1 M NaoH, 6 M guanidine hydrochloride, 8 M urea. alcohol upto 30% v/v concentartion.
Storage:20% ethanol, 4°C

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