G-Sep™ Ni NTA Agarose Fast Flow, 100ml, 786-1042, G-BIOSCIENCES (CAT#: STEM-C-3177-LGZ)

Immobilized metal ion affinity chromatography (IMAC) was developed by Porath (1975) and is based on the association of certain protein residues (histidine, cysteine and to some extent tryptophan) with transition metals cation interactions. G-Sep™ NI NTA Agarose Fast Flow is designed for purification of recombinant proteins fused to a 6x Histidine (6XHis) tag.

This resin is specifically designed for the purification of recombinant proteins fused to a 6X histidine (6XHis) tag expressed in bacterial, insect, and mammalian cells. The resin has high affinity and selectivity for recombinant fusion proteins tagged with 6 tandem histidine residues.

G-Sep™ NI NTA Sepharose Fast Flow (FF) Resin is nickel ions immobilized on highly cross-linked 6% agarose beads. G-Sep™ NTA Sepharose Fast Flow (FF) Resin is highly chemically stable, allowing for well-validated cleaning-in-place (CIP) and sanitation protocols. G-Sep™ Ni NTA Agarose Fast Flow (FF) Resin using Cobalt is also available.

Size: 100ml
Matrix: Cross-linked agarose beads, 6%
Bead form: Spherical, diameter 50-160μm
Spacer: Epichlorohydrin
Chelating Agent: nitrilotriacetic acid (NTA)
pH stability Working Range: 3-12
pH stability Cleaning-in-Place (CIP): 2-14
Maximum Flow Velocity: 450cm/h
Exclusion limit(globular proteins): 4 x 106
Physical Stability: Negligible volume variation due to changes in pH or ionic strength
Chemical Stability: Stable to all commonly used aqueous buffers: 6M urea, 8M guanidine hydrochloride,
Autoclavable: 121°C, pH 7, for 30 min
Storage Conditions: 2 to 8°C, 20% Ethanol

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