G-Trap™ CM Agarose Fast Flow, 5 ml, 5 Columns, 786-1011, G-BIOSCIENCES (CAT#: STEM-C-3308-LGZ)

G-Trap™ CM Agarose Fast Flow is ready-to-use prepacked columns for protein and other charge-based biomolecular separations. The resin in G-Trap™ CM Sepharose Fast Flow is a weak cation exchanger consisting of highly cross-linked 6% agarose beads with carboxymethyl (CM) weak cation exchange groups. Proteins with a net positive charge will bind to the resin, while proteins with a net negative charge will not bind and will flow out.

There are other G-Trap™ Ion Exchange Agarose Fast Flow columns available in 1 mL and 5 mL column volumes, namely G-Trap™ SP Agarose Fast Flow, G-Trap™ DEAE Agarose Fast Flow and G-Trap™ Q Agarose fast flow. G-Trap™ SP Sepharose Express contains cation exchange resin and G-Trap™ DEAE Sepharose Express and G-Trap™ Q Sepharose Express contains anion exchange resin. The G-Trap™ Ion Exchange Selection Kit can be used for initial screening of suitable ion exchangers for purification of target proteins.

G-Trap™ Ion Exchange Agarose Fast Flow Columns can separate biomolecules on the basis of charge using prepacked columns. G-Trap™ Ion Exchange Agarose Fast Flow Columns are used for method detection, separation or purification of biomolecules, sample concentration and sample cleanup of charged molecules. Biomolecules carrying a net charge opposite to the ion exchanger bind to the ion exchanger and are thus separated from the bulk molecules in the sample.

Size: 5 columns

G-Trap™ CM Agarose Fast Flow resin

Ion Exchanger: Weak cation exchanger
Matrix: Cross-linked agarose beads, 6%
Bead size: 45-165 µm
Ligand: Carboxymethyl
Ionic Capacity: 0.09-0.13 mmol (H+)/ml
Binding Capacity: 50 mg lysozyme/ ml medium
pH stability Working Range: 4-12
pH stability Cleaning-in-Place (CIP): 2-14
Maximum Flow Velocity: 450cm/h
Recommended Flow rate: 1 ml/ min and 5 ml/ min for 1 ml and 5 ml column respectively
Maximum Pressure: 0.3MPa
Exclusion limit(globular proteins): 4 x 106
Chemical Stability: Stable to all commonly used aqueous buffers:1 M NaOH, 8M urea, 8M guanidine hydrochloride, 70% ethanol
Storage Conditions: 4 to 30°C, 20% Ethanol


G-Trap™ CM Agarose Fast Flow​, 1 ml columns
Column Volume: 1 ml

Columns Dimension: 0.7 x 2.5 cm
Column Harware Pressure Limit: 0.5 MPa
Column Hardware: Polypropylene (Biocompatible)

G-Trap™ CM Agarose Fast Flow​, 5 ml columns

Column Volume: 5 ml
Columns Dimension: 1.6 x 2.5 cm
Column Harware Pressure Limit: 0.5 MPa
Column Hardware: Polypropylene (Biocompatible)

Spin Column, 10ml, 10 Columns, 786-727, G-BIOSCIENCES (CAT#-STEM-C-3328-LGZ)
Spin Column, 10ml, 25 Columns, 786-983, G-BIOSCIENCES (CAT#-STEM-C-3329-LGZ)
Spin Column, 10ml, 50 Columns, 786-984, G-BIOSCIENCES (CAT#-STEM-C-3330-LGZ)
Spin Column, 1ml, 25 Columns, 786-810, G-BIOSCIENCES (CAT#-STEM-C-3331-LGZ)
Spin Column, 1ml, 50 Columns, 786-811, G-BIOSCIENCES (CAT#-STEM-C-3332-LGZ)
Twist Break Column, 25 Columns, 786-1810, G-BIOSCIENCES (CAT#-STEM-C-3333-LGZ)