G-Trap™ Ni IDA Agarose Fast Flow, 1 ml, 5 Columns, 786-1021, G-BIOSCIENCES (CAT#: STEM-C-3179-LGZ)

G-Trap™ Ni IDA Sepharose Fast Flow Columns are ready-to-use prepacked affinity columns for the purification of proteins based on immobilized metal ion affinity chromatography (IMAC).

IMAC is formed by the interaction of transition metal cations such as Ni2+ and Co2+ with protein residues histidine, cysteine and to some extent tryptophan. In G-Trap™ Ni IDA Agarose Fast Flow, Ni2+ is immobilized on cross-linked 6% agarose through iminodiacetic acid groups (IDA) covalently bound to the resin.

G-Trap™ Ni IDA Agarose Fast Flow is designed for the purification of recombinant proteins fused to a 6-fold histidine (6XHis) tag.

Additional G-Trap™ IDA Agarose Fast Flow columns are available in 1 mL and 5 mL column volumes for G-Trap™ Co IDA Agarose Fast Flow. You can choose between the appropriate columns according to your needs.

Size: 5 columns

G-Trap™ Ni IDA Agarose Fast Flow resin
Matrix: Cross-linked agarose beads, 6%
Mean particle size: 90 μm
Chelating Agent: Iminodiacetic acid
Active group: Ni2+
Metal ion density: 20-40 μmol /ml
Binding Capacity: 5-10mg His-tagged protein/ml medium
pH stability Working Range: 3-12
pH stability Cleaning-in-Place (CIP): 2-14
Maximum Flow Velocity: 2-14
Recommended Flow rate: 1 ml/min and 5 ml/min for 1 ml and 5 ml column respectively
Exclusion limit(globular proteins): 4 x 106
Chemical Stability: Stable to all commonly used aqueous buffers: 8 M urea, 6 M guanidine hydrochloride
Storage Conditions: 2 to 8°C, 20% Ethanol

G-Trap™ Ni IDA Agarose Fast Flow​, 1 ml columns
Column Volume: 1 ml
Columns Dimension: 0.7 x 2.5 cm
Column Harware Pressure Limit: 0.5 MPa
Column Hardware: Polypropylene (Biocompatible)

G-Trap™ Ni IDA Agarose Fast Flow, 5 ml columns
Column Volume: 5 ml
Columns Dimension: 1.6 x 2.5 cm
Column Harware Pressure Limit: 0.5 MPa
Column Hardware: Polypropylene (Biocompatible)

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