G-Trap™ rProtein A FF is prepackaged and ready for column purification of IgG antibodies. Recombinant protein A is covalently coupled to highly cross-linked 6% agarose using a proprietary conjugation method. Recombinant protein A produced in E. coli has been genetically modified to enhance its ability to bind antibodies G-trap™ Protein G, 1ml and G-trap™ Protein G, 5ml can also be used for affinity purification of IgG.
Specification
Size: 5 columns
Features
G-Trap™ rProtein A resin
Mean particle size: 90 µM Bead Structure: Highly cross-linked 6% agarose Ligand: Recombinant Staphylococcal Protein A from E.coli Binding capacity:>40 mg Human IgG/ ml resin Recommended working flow rate:1 ml/min and 5 ml/min for 1 ml and 5 ml column respectively. Chemical Stability:Stable in all commonly used buffers pH stability: Long term: pH 3-9, short term pH 2-9 Storage: 2°C to 8°C in 20% ethanol
G-Trap™ rProtein A, 1 ml columns
Column Volume: 1 ml Columns Dimension: 0.7 x 2.5 cm Column Harware Pressure Limit: 0.5 MPa Column Hardware: Polypropylene (Biocompatible)
G-Trap™ rProtein A, 5 ml columns
Column Volume: 5 ml Columns Dimension: 1.6 x 2.5 cm Column Harware Pressure Limit: 0.5 MPa Column Hardware: Polypropylene (Biocompatible)
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