Gel Filtration PhyTip® Columns (1000 µL Volume) Containing 200 µL Of 5K Size Exclusion Resin Per Column, PDR-91-20-06 (CAT#: STEM-C-4576-LGZ)

Gel Filtration PhyTip Column Packed Size Exclusion Chromatography Resins are ideal for desalted protein samples and buffer exchange. In the same automated protocol, gel filtration PhyTip column desalting can be used as a secondary purification step after affinity purification, such as imidazole removal after IMAC affinity purification.

Just like other PhyTip columns, the resin is packed between two inert barriers to maintain a resin bed at the bottom of the column. However, unlike other PhyTip columns, gel filtration columns are top loaded with sample; gravity flow pulls the sample through the gel. Gel Filtration PhyTip columns are available with 5K and 30K size exclusion resins, which retain small molecules when molecules over 5kDa and 30kDa pass through the column, respectively.

Pack Size: 96

Biotage Gel Filtration PhyTip® Columns enable researchers to prepare and process large numbers of potential antibody and protein leads by providing fully automated desalting and buffer exchange solutions.

High Purity and Sample Recovery
Gel filtration PhyTip columns remove 95% of salt and 80% of protein yield.

Part of a complete automation protocol
Gel Filtration PhyTip Columns can be easily combined with Affinity PhyTip Columns for protein purification in the same automated workflow.

consistent results
Our automated solutions provide highly consistent and reproducible results with sample volumes and concentrations of 10% cv between purified samples while maintaining protein functionality.

Speed
Gel Filtration PhyTip columns can process up to 96 samples in approximately 30 minutes, much faster than overnight dialysis buffer exchange.

Compatible with any liquid handling system
Our Gel Filtration PhyTip columns are compatible with the MEA 2 system or any other liquid handling system with a clamping arm. The gripper arm can be used to move the tray as needed for desalting, buffer exchange, or fractional capture when screening 96 samples in the same run.

Ion Exchange PhyTip® Columns (300 µL Volume) Containing 5 µL Weak Cation Exchange Resin Per Column, PTH-93-05-14, Hamilton (CAT#-STEM-C-4570-LGZ)
Ion Exchange PhyTip® Columns (500 µL Volume) Containing 160 µL Weak Cation Exchange Resin Per Column, PTT-95-16-14, Tecan (CAT#-STEM-C-4571-LGZ)
Ion Exchange PhyTip® Columns (500 µL Volume) Containing 20 µL Weak Cation Exchange Resin Per Column, PTT-95-20-14, Tecan (CAT#-STEM-C-4572-LGZ)
Ion Exchange PhyTip® Columns (500 µL Volume) Containing 40 µL Weak Cation Exchange Resin Per Column, PTT-95-40-14, Tecan (CAT#-STEM-C-4573-LGZ)
Ion Exchange PhyTip® Columns (500 µL Volume) Containing 80 µL Weak Cation Exchange Resin Per Column, PTT-95-80-14, Tecan (CAT#-STEM-C-4574-LGZ)
Gel Filtration PhyTip® Columns (1000 µL Volume) Containing 200 µL Of 30K Size Exclusion Resin Per Column, PDR-91-20-17 (CAT#-STEM-C-4575-LGZ)