Genomic Library and cDNA Library

A DNA library is a collection of DNA fragments cloned into vectors to recognize and usually used to isolate desired DNA fragments, which is convenient for researchers to study interesting genes. In this technique, the fragments of total DNA are inserted into vectors randomly and then transformed into host cells, and the genes are cloned along with the cellular proliferation.

There are two types of DNA library – genomic library and cDNA library. The genomic DNA libraries comprise the total genomic DNA from a single organism, while cDNA libraries are only constituted by reverse-transcribed mRNA without the unexpressed DNA sequences such as the introns.

Genomic Library

A genomic library is a collection of the total genomic DNA from a single organism. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.

cDNA Library

A cDNA library is a combination of cloned cDNA fragments inserted into a collection of host cells. cDNA is produced from fully transcribed mRNA found in the nucleus and therefore contains only the expressed genes of an organism.

Key Difference between Genomic library and cDNA library

	Genomic library	cDNA library
Definition	Collection of clones having the complete genomic DNA of a single organism	Collection of clones having complementary DNA to the mRNA of a single organism
Contains	All genes	Genes expressed in a particular cell at a given period
Size	Larger	Smaller
Enzyme required	Ligases and restriction endonucleases	Reverse transcriptase enzyme
Start with	DNA	mRNA
Expression	Gene expression extracted from the genomic library is challenging in the prokaryotic systems, as they are devoid of a splicing mechanism	Can be directly expressed as it has only coding sequences

STEMart provides you with a variety of equipment or consumables used in the research of DNA library to meet your various R&D and application needs. If you have any questions or requirements for genomic library and cDNA library, please feel free to contact us.

Genomic Library and cDNA Library

Genomic Library

cDNA Library

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