Glutathione PhyTip® Columns (200 µL Volume) Containing 5 µL Affinity Media Per Column, PTB-92-05-04, Beckman (CAT#: STEM-C-4359-LGZ)

Glutathione PhyTip columns are used for automated purification of GST-tagged antibodies and other recombinant proteins. The affinity resins used in these columns exhibit high binding affinity and affinity purification of functional recombinant GST-tagged proteins. The GST-tag (glutathione s-transferase) is a small (26 kDa) protein known to increase the solubility of expressed recombinant proteins.

Our Glutathione PhyTip columns come with a validated DFC protocol for the purification of GST-tagged proteins and can be transferred to any automated liquid handling system. Our buffer kit contains all the buffers needed for the purification protocol. Note that a slow flow rate of 0.06 mL/min is required for proper use of Glutathione PhyTip columns.

Pack Size: 96

PhyNexus, now part of Biotage, developed the PhyTip column in 2002 and has supported researchers in automated protein purification since then. PhyTip columns purify samples through repeated back-and-forth cycles, a process called dual-flow chromatography that allows binding interactions to be completed during each purification step.

90% sample purity
Glutathione PhyTip columns continuously produced 90% pure samples even in the presence of 40x excel BSA.

60 - 80% sample recovery
Glutathione PhyTip columns typically yield purified samples with high sample recovery.

Microscale high-throughput parallel purification
The PhyTip column format is the perfect tool for parallel processing of microscale samples in high-throughput processes.

Compatible with all major liquid handling systems
Our PhyTip columns are available for all major liquid handling systems, allowing them to be used on existing instruments you already have in your lab!

15 minute protein cleanse
Efficient purification via PhyTip columns allows for a complete purification protocol of 96 samples in as little as 15 minutes.

Validated Protocols
Our Protein A PhyTip columns use dual-flow chromatography, are true protein validated, and can be used in any automated liquid handling system.

Glutathione PhyTip® Columns (1 mL Volume) Containing 80 µL Affinity Media Per Column, PTH-91-80-04, Hamilton (CAT#-STEM-C-4353-LGZ)
Glutathione PhyTip® Columns (1 mL Volume) Containing 80 µL Affinity Media Per Column, PTR-91-80-04, MEA 2 (CAT#-STEM-C-4354-LGZ)
Glutathione PhyTip® Columns (1 mL Volume) Containing 80 µL Affinity Media Per Column, PTT-91-80-04, Tecan (CAT#-STEM-C-4355-LGZ)
Glutathione PhyTip® Columns (200 µL Volume) Containing 20 µL Affinity Media Per Column, PTB-92-20-04, Beckman (CAT#-STEM-C-4356-LGZ)
Glutathione PhyTip® Columns (200 µL Volume) Containing 20 µL Affinity Media Per Column, PTR-92-20-04, MEA 2 (CAT#-STEM-C-4357-LGZ)
Glutathione PhyTip® Columns (200 µL Volume) Containing 20 µL Affinity Media Per Column, PTT-92-20-04, Tecan (CAT#-STEM-C-4358-LGZ)