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Glutathione S-Transferase Analysis in Plant Tissues

All kinds of abiotic and biotic stresses significantly limit plant productivity by disturbing cellular biochemistry and physiology via the overproduction of activated oxygen molecule-reactive oxygen species (ROS). Uncontrolled accumulation of ROS results in oxidation of DNA and RNA, protein denaturation and decreased enzyme activity as well as lipid peroxidation and carbohydrate oxidation.

Glutathione S-transferase (GST) is a family of enzymes with important functions in plant detoxification and resistance to pathogen infections. At the same time, as an important ROS-scavenging enzyme, GST catalyzes the reaction of electrophilic substrates to remove overproduced ROS under biotic and abiotic stress conditions, such as high salt, hypoxia, osmotic dehydration, and exposure to safeners and oxylipins.

STEMart provides comprehensive analysis services to identify and investigate the function of GST in regulating plant environmental stress responses.

Test Samples

Plant tissue

Test Capabilities

  • Identification of GST genes
  • Phylogenetic analysis of GST genes
  • Structure analysis of GST genes
  • Expression profile of GST genes of plant under stress conditions
  • 3D structure and physicochemical properties of GST genes
  • Subcellular localization of GST enzymes and Isoenzymes analysis
  • Activity determination of GST

Why Choose Us

  • Comprehensive test report including relevant experimental steps, relevant parameters of the experiment, original pictures, original data, and complete experiment results
  • Customized service for plant research
  • Professional instruments and equipment for reliable data and results
  • Rapid turnaround and cost-effective

For more information about our plant GST analysis service, please contact us.

Reference

  1. Du B., Zhao W., An Y., Li Y., Zhang X., Song L., Guo C. (2019). “Overexpression of an alfalfa glutathione S-transferase gene improved the saline-alkali tolerance of transgenic tobacco”. Biol. Open. 8.
  2. Roxas V.P., Lodhi S.A., Garrett D.K., Mahan J.R., Allen R.D. (2000). “Stress tolerance in transgenic tobacco seedlings that overexpress glutathione S-transferase/glutathione peroxidase”. Plant Cell Physiol. 41:1229–1234.

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