GPCR Pathway Assay (CAT#: STEM-MB-0325-WXH)

Introduction

G protein-coupled receptors (GPCRs) are a large family of cell surface transmembrane receptors. More than 800 members have been discovered. They are involved in a variety of life activities and various physiological processes such as endocrine and metabolism. The occurrence, development and treatment of important diseases such as, tumors and immune diseases are closely related. Based on the important biological role of GPCRs in physiological and pathological processes, this protein family has now become the most important drug target library.
G protein-coupled receptors are only found in eukaryotes and are involved in many cell signal transduction processes. In these processes, G protein-coupled receptors can bind to chemicals in the surrounding environment of the cell and activate a series of signaling pathways in the cell, ultimately causing changes in the cell state.

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Principle Applications Procedure Materials Notes Related Products

Principle

GPCR regulate a variety of normal biological processes, and play a role in the pathophysiology of many diseases after dysregulation of downstream signal activities. The intracellular signaling pathways activated by GPCR signals include cAMP/PKA pathway, PKC pathway, Ca2+/NFAT pathway, PLC pathway, PTK pathway, PKC/MEK pathway, MAPK pathway, p38MAP pathway, PI3K pathway, Rho pathway, NF-κB pathway and JAK/STAT pathway. GPCR regulates a variety of biological processes, such as vision, smell, autonomic nervous system, and behavior. GPCR recognizes various ligands and stimuli, including peptide and non-peptide hormones and neurotransmitters, chemokines, prostaglandins and proteases, biogenic amines, nucleosides, lipids, growth factors, odor molecules, and light. The development of drug screening assays requires investigation of the target and related GPCR expression and function in the selected cell-based model system, as well as the expression of related GPCRs, to assess direct and potential off-target side effects.

Applications

To study the regulation mechanism of GPCR signal pathway in disease
To study the effect of each virus on GPCR signaling pathway
To study the effects of drugs or therapies on GPCR signaling pathways

Procedure

• Luminex Multiplex Assay
• Enzyme-linked immunosorbent assay (ELISA)
• Flow cytometry (FACS analysis) technology

Notes

Detectable targets: JNK3, PI3K, PKC, STAT1, STAT2, STAT3, EPAC, IRAK4, JNK, MYD88, RTKs, TLR4, CREB, IRF3, MAPK, NAP1, Rel, TLR9, TCR, icam1, Mda-5, NFκB, RIG-1, TRAF3, GAS, IRF9, MEKK1, p38, RIP1, TRAF5, CD3, IRF5, MEK3, p38MAPK, SH2, TRAF6, PKA, IRS1, MEK6, p50, SLP76, TRAM, PSGL1, IRS2, MSK1, TLR3, Tak1, TRIF, TNFR, ISGF3, MSK2, PI3K, TBK1, Vav, Fas, ISRE, mTOR, PKR

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