High-fidelity PCR (CAT#: STEM-MB-0204-WXH)

Introduction

High-fidelity PCR is a modifies PCR method that utilizes a DNA polymerase with a low error rate and results in a high degree of accuracy in the replication of the DNA of interest.
Such enzymes have a significant binding affinity for the correct nucleoside triphosphate during amplification.
In the case of an incorrect binding in the polymerase active site, incorporation is slowed due to the architecture of the active site complex.
Highfidelity amplification is essential for experiments whose outcome depends upon the correct DNA sequence like cloning, SNP analysis, NGS applications.