His-Tagged Protein Purification Medium (NTA), Agarose Gel Microspheres, Bogen (CAT#: STEM-C-3005-LGZ)

The His-tagged protein purification medium is suitable for the purification of His-tagged fusion proteins. This product can quickly extract the His-tagged fusion protein from cell fermentation broth.

Matrix: Agarose gel microspheres
Ligand: NTA
Shape: spherical
Average particle size of medium: 90±5 μm
Dynamic capacity: ≥20 mg His-Pro/ml wet gel (NTA)
Reducing agent tolerance concentration: β-mercaptoethanol≤100mM, DTT≤5mM
Service life: 100-150 times (depending on the clarity of the protein solution)
Maximum flow rate (25°C): ≥500 cm/h
Maximum pressure: 0.3 MPa (3 bar)
Storage: 4~8 °C (20% ethanol)

His-tagged protein purification medium (NTA) uses NTA that can withstand reducing agents as metal chelating ligands, conventionally chelating Ni2+ (often called nickel filler), and can also chelate Cu2+, Co2+, Zn2+ plasma groups according to customer needs .

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