His60 Ni Gravity Columns, 5 x 1 mL Columns, 635657, Takara Bio Inc (CAT#: STEM-C-3826-LGZ)

His60 Ni Superflow Resin is a high-capacity Ni-ida resin for the one-step purification of recombinant his-tagged proteins from bacterial, mammalian, and baculovirus-infected cells. His60 Ni resin is compatible with batch/gravity flow applications, as well as major automated LC systems and manual syringe handling. The resin allows for fast, simple, reproducible chromatographic separations and can be regenerated for multiple uses.

His60 Ni gravity columns are prepacked with 1 ml of resin. The His60 Ni Gravity Column Purification Kit provides all the buffers needed for protein extraction and purification, plus a pre-packaged His60 Ni Gravity Column. These buffers are also available individually in the His60 Nickel Buffer Set.

Purification principle of recombinant his tagged protein

His60 Ni superfluid purification resin has low metal ion leakage and the highest binding capacity so far (better than resins such as Ninta). The modified Ni-IDA chemistry makes this resin one of the best on the market in terms of capacity, metal ion leakage and purity when compared to other nickel-based resins such as Ni-NTA or other Ni-IDA resins.

Purification process of recombinant his-tagged protein

Purification of recombinant His-tagged proteins requires cell lysis/extraction, binding, washing and elution. His60 Ni makes protein purification under native or denaturing conditions a one-step process. The resin is compatible with a wide range of denaturants and detergents. Purified recombinant His-tagged proteins can be eluted by adding 300 mM imidazole or lowering the pH.

Size: 5 x 1 mL Columns

Obtain up to 60 mg/ml of recombinant his-tagged protein
Low leakage of metal ions in the elution of his-tagged proteins
Purified under native or denaturing conditions
For batch/gravity flow and automated FPLC cleanup

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