Histological diagnostics of early stages of ischaemic and metabolic myocardial lesions by polarized light microscopy (CAT#: STEM-MIT-0210-LJX)

Introduction

Cardiomyocytes and muscle fibers, with stripes. Innervated by vegetative nerves, it is an involuntary muscle with transverse lines, capable of excitatory contractions. It is short cylindrical and has branches, and its nucleus is located in the center of the cell, usually only one. The ends of each myocardial fiber branch can be connected to form a network of muscle fibers.
As basic types of myocardial cell lesions there can be distinguished segmental and subsegmental contractures, intracellular myocytolysis, and primary granular disintegration. Animal experiments have proved that the earliest morphological manifestations of acute lesions of myocardial cells (at the optical microscopic level) are myofibrillar changes detectable by polarized-light microscopy.

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Principle Applications Procedure Materials Notes Related Products

Principle

Polarized light microscopy is a kind of microscope to identify the optical properties of the fine structure of a substance. Any substance with birefringence can be distinguished under a polarizing microscope. The characteristic of a polarized light microscopy is to change ordinary light into polarizing light for microscopic examination in order to identify whether a substance is monorefringent (anisotropic) or birefringent (anisotropic).
1. Monorefractivity and birefringence: When light passes through a substance, if the properties and path of light do not change due to the direction of illumination, this substance has "isotropy" in optics, also known as a single refractor, such as ordinary gases, liquids, and amorphous solids; If the speed, refractive index, absorption, polarization, amplitude, etc. of light passing through another material vary depending on the direction of illumination, this material has "anisotropy" in optics, also known as a birefringent material, such as crystals, fibers, etc.
2. Polarization of light: Light waves can be divided into natural light and polarized light according to the characteristics of vibration. The vibration characteristic of natural light is that there are many vibration planes on the vertical light wave conduction axis, and the amplitude distribution of vibration on each plane is the same. Natural light through reflection, refraction, birefringence and absorption, can be obtained only in one direction vibration of light waves, this light wave is called "polarized light" or "polarized light".

Applications

Study and analysis in mineral, chemistry, biology, botany and other fields

Procedure

1. Sampling
2. Preparation of slices
3. Staining (Select according to the specific experimental situation)
4. Observation

Materials

• Sample Type:
Cardiomyocyte

Notes

Operate in strict accordance with the operating procedures, and shall not arbitrarily change the operating procedures

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