Histone Methylation Analysis Service Based on Flow Cytometry Systems

STEMart offers specialized Histone Methylation Analysis services using advanced flow cytometry systems. These systems are critical to our laboratory's analytical capabilities, enabling precise detection and quantification of histone modifications at the single-cell level. Histone methylation plays a crucial role in the regulation of gene expression, chromatin structure, and cellular identity, making it a key focus area in epigenetics, cancer research, and developmental biology.

Flow cytometry systems used for histone methylation analysis leverage fluorescently labeled antibodies that specifically recognize methylated histones. By passing cells through a laser beam, the flow cytometer detects and quantifies the fluorescence intensity, which correlates with the level of histone methylation in individual cells. This method allows for high-throughput analysis of histone modifications, providing insights into epigenetic regulation across different cell types and conditions.

Leveraging our state-of-the-art flow cytometry systems, we offer a range of comprehensive Histone Methylation Analysis services. These services are tailored to meet your specific research needs, ensuring accurate and detailed epigenetic profiling of your samples.

What We Offer

• Global Histone Methylation Profiling

Comprehensive analysis of global histone methylation patterns across various cell types, providing insights into overall epigenetic regulation.

• Site-Specific Histone Methylation Analysis

Targeted analysis of specific histone modifications, such as H3K4me3 or H3K27me3, to study their role in gene regulation and cellular function.

• Comparison of Histone Methylation Across Conditions

Comparative analysis of histone methylation levels between different experimental conditions, such as treatment vs. control, to identify epigenetic changes associated with specific interventions.

• Multiparametric Analysis

Integration of histone methylation data with other cellular parameters, such as cell cycle status or surface marker expression, for a comprehensive understanding of cellular states.

Advantages of Services Using Our Flow Cytometry Systems

• High Sensitivity and Specificity

Our flow cytometry systems offer high sensitivity in detecting subtle changes in histone methylation, ensuring accurate and reliable data.

• Single-Cell Resolution

Analyze histone methylation at the single-cell level, allowing for the identification of heterogeneous epigenetic states within cell populations.

• High Throughput Capability

Capable of processing large sample sizes quickly, making it ideal for studies requiring extensive data collection and statistical analysis.

• Versatility in Assay Design

Customizable assays tailored to specific histone marks and research questions, providing flexibility and precision in epigenetic studies.

Our Equipment

BD FACSymphony A5

A high-parameter flow cytometer with the capability to detect multiple histone modifications simultaneously, providing detailed epigenetic profiles at the single-cell level.

Cytek Aurora

A spectral flow cytometer that offers enhanced resolution and sensitivity, ideal for detecting subtle differences in histone methylation across cell populations.

BD LSRFortessa

A versatile flow cytometer that supports high-dimensional analysis, widely used for comprehensive studies of histone modifications and other epigenetic markers.

BD FACSAria Fusion

Combines cell sorting with multiparametric analysis, enabling the isolation of cells based on specific histone methylation patterns for downstream applications.

STEMart provides high-quality flow cytometry services tailored to meet the specific needs of our clients. Our experienced specialists use advanced equipment and follow strict quality control measures to ensure accurate and reliable results. Contact us to learn more about our flow cytometry services and how we can help you achieve your research goals.