Homogeneity of viral RNA molecules by Dynamic light scattering (DLS) (CAT#: STEM-MB-0539-WXH)

Introduction

RNA molecules are highly labile, since exposure to trace amounts of ribonuclease impurities present in aqueous solutions and reagents, air and dust particles, as well as direct human skin contact with RNA preparations, can result in RNA degradation. Dynamic light-scattering instrument is regularly used to study homogeneity of in-vitro transcribed RNA.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Dynamic Light Scattering (DLS) is an established and precise measurement technique for characterizing particle sizes in suspensions and emulsions. It is based on the Brownian motion of particles - this states that smaller particles move faster, while larger ones move slower in a liquid. The light scattered by particles contains information on the diffusion speed and thus on the size distribution.
The Dynamic Light Scattering (DLS) technique measures motion optically by recording the scattered light signal at a fixed angle. The particles are illuminated with a monochromatic, coherent light source (laser) and the light scattered by the particles is recorded.

Applications

DLS is used to characterize the size of various particles including proteins, polymers, micelles, Protein cages and virus-like particles, vesicles, carbohydrates, nanoparticles, biological cells, and gels.

Procedure

1. Sample preparation
2. Measurement by DLS instrument
3. Data analysis

Materials

• Dynamic Light Scattering (DLS) instruments (Photon Correlation Spectroscopy or Quasi-Elastic Light Scattering instruments)
• Dynamic Light Scattering Detector

Other recommended products

Homogeneity studies of Ovotransferrin by Dynamic light scattering (DLS) (CAT#: STEM-MB-0536-WXH)
Nanosize Particle Analysis by Dynamic light scattering (DLS) by Dynamic light scattering (DLS) (CAT#: STEM-MB-0548-WXH)
Analysis of the effect of Mg2+ on myosin filament polymerization/depolymerization by Dynamic light scattering (DLS) (CAT#: STEM-MB-0550-WXH)
Homogeneity studies of Aprotinin v (CAT#: STEM-MB-0538-WXH)
Analysis of Protein–protein interaction by Dynamic light scattering (DLS) (CAT#: STEM-MB-0540-WXH)
Homogeneity studies of Thyroglobulin by Dynamic light scattering (DLS) (CAT#: STEM-MB-0533-WXH)
Tracing protein aggregates by Dynamic light scattering (DLS) (CAT#: STEM-MB-0549-WXH)
Homogeneity studies of nidogen-1 (ND-1) by Dynamic light scattering (DLS) (CAT#: STEM-MB-0531-WXH)