HOOK™ GST Protein Spin Purification Kit (Yeast), Size 25, 786-642, G-BIOSCIENCES (CAT#: STEM-GT-3012-LGZ)

HOOK™ GST Protein Spin Purification Kit provides rapid purification of soluble GST-tagged proteins from yeast culture. Yeast is first lysed with Yeast PE LB™ and LongLife™ Zymolyase® to release total soluble protein while maintaining protein structure and activity. GST-tagged proteins were purified by affinity chromatography by adding 0.5 ml glutathione resin to the clarified lysate. The resin is transferred to a convenient spin column where it is quickly washed and the GST protein is eluted.

Yeast-pe LB™ is used to extract soluble proteins from yeast cells. Yeast PE LB™ is a patented improvement to Zymolyase®-based spheroplast preparation and extraction of soluble proteins from yeast cells. Yeast PE LB™ is based on organic buffers utilizing mild non-ionic detergents and a proprietary combination of various salts and agents to enhance protein extraction and stability. A ready-to-use formulation of Zymolyase® is also provided. Depending on the application, other agents such as protease inhibitors can be added to Yeast PE LB™. This proprietary combination of reagents provides a simple and versatile method for yeast protein extraction. Yeast PE LB™ eliminates the need for glass bead lysis of yeast cells.

The HOOK™ GST Protein Spin Purification Kit is optimized to yield ~1 mg of soluble GST-tagged protein with a purity of 80-90%, depending on the protein's expression level, resin type, conformation, and solubility characteristics.

For large-scale isolation of GST proteins from yeast, we recommend our gravity flow kit to extract 10mg of protein from yeast.

Size: 25

Isolate >1mg GST tagged proteins from ~50ml yeast culture
No sonication required, supplied with lysis buffer and enzyme
Spin column format

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