Hybrid Fusion FISH (HF-FISH) (CAT#: STEM-MB-1180-WXH)

Introduction

HF-FISH technology uses a homogenous complex of differentially-labeled probe DNA and polyanionic glucan polysaccharide derivatives such that the complex is designed to recognize a particular target DNA sequence for genetic interrogation.

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Principle Applications Procedure Materials Notes Related Products

Principle

Hybrid Fusion FISH (HF-FISH) uses primary additive excitation/emission combination of fluorophores to generate additional spectra through a labeling process known as dynamic optical transmission (DOT). Three primary fluorophores are able to generate a total of 7 readily detectable emission spectra as a result of combinatorial labeling using DOT. Hybrid Fusion FISH enables highly multiplexed FISH applications that are targeted within clinical oncology panels. The technology offers faster scoring with efficient probesets that can be readily detected with traditional fluorescent microscopes.

Applications

Detecting genetic aberrations

Materials

• Flow cytometer
• Fluorescence microscopy

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