IL-10 Detection (CAT#: STEM-MB-0279-WXH)

Introduction

IL-10 is a multi-effect inhibitory cytokine, which can be produced by monocytes, macrophages, dendritic cells, B cells, CD8 + T cells, regulatory T cells, Th1 cells, and Th17 cells. Others Some non-immune cells (mast cells, keratinocytes, epithelial cells and tumor cells). IL-10 can inhibit the activation of T cells, monocytes and macrophages, and inhibit the production of various cytokines. It can also promote the differentiation and proliferation of B cells and natural killer cells, and promote the recruitment of monocyte nuclear macrophages and mast cell colonies. In addition, IL-10 plays an important role in various diseases such as inflammatory response, autoimmune disease, tumor and organ transplantation.

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Principle Applications Procedure Materials Notes Related Products

Principle

The IL-10 receptor is composed of two a chains and two b chains. Both IL-10RA and IL-10RB belong to the type II cytokine receptor family. IL-10RB is widely expressed and expressed in most cells and tissues. The IL-10 signal works mainly through the JKA-STAT pathway. A tetramer formed by the combination of IL-10 with two A chains and two B chains, leading to activation of JAK1 and TYK2, and causing phosphorylation of specific parts of the IL-10 receptor terminal by JAK1. The phosphorylated receptor causes aggregation and connection of STAT3, and the connected STAT3 is phosphorylated by JAK1 and TYK2 to form a dimer, which regulates the expression of corresponding target genes.

Applications

IL-10 can inhibit the activation of T cells, monocytes and macrophages, and inhibit the production of various cytokines.
IL-10 can also promote the differentiation and proliferation of B cells and natural killer cells
IL-10 can promote the recruitment of monocyte nuclear macrophages and mast cell colonies.
IL-10 plays an important role in various diseases such as inflammatory response, autoimmune disease, tumor and organ transplantation.

Procedure

1. Process samples.
2. IL-10 detection (qPCR, Enzyme-linked immunosorbent assay (ELISA), Flow cytometry).
3. Analysis results.

Notes

Sample Types-Blood, serum, plasma, cerebrospinal fluid, cell culture supernatant, tissue homogenate, cell culture medium, urine, tumor, etc.

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