IL-20 Detection (CAT#: STEM-MB-0287-WXH)

IL-20 is a soluble protein and belongs to the IL-10 cytokine family. IL-20 is secreted by activated keratinocytes and monocytes, and transmits signals to the cell through two receptors expressed on the surface of other endothelial cells. IL-20 has certain homology with IL-10 and other IL-10 family members in the amino acid sequence, such as 28% homology with IL-10. There are 6 conserved cysteine residues in the IL-20 molecule, of which cys81 and cys134 form a disulfide bond, but do not form a dimer. IL-20 can play a role by binding to the receptor, and two subunits must exist at the same time to efficiently bind to the ligand. IL-20 receptors (IL-20Rα/IL-22Rα, IL-20Rβ) are expressed in renal tissue interstitial fibroblasts, renal epithelial cells, mesangial cells and podocytes. After IL-20 binds to the receptor, it activates STAT3 by binding to JAK1 and TYK2. The two STAT3 form a homodimer through the nuclear membrane to enter the nucleus, regulate gene expression, and produce biological effects.

IL-20 can enhance inflammation, angiogenesis and chemotaxis.
IL-20 can regulate the normal physiological functions of skin.
IL-20 can regulate the occurrence of early skin inflammation.
IL-20 can promote the proliferation and differentiation of keratinocytes.
IL-20 can promote the secretion of cytokines and chemokines.

1. Process samples.
2. IL-20 detection (qPCR, Enzyme-linked immunosorbent assay (ELISA), Flow cytometry).
3. Analysis results.

Sample Types-Blood, serum, plasma, cell culture medium, tissue homogenate, etc.