IL-21 Detection (CAT#: STEM-MB-0288-WXH)

IL-21R is a complex composed of a unique IL-21R subunit and γc (γ-chain). Among them, the IL-21R subunit is the ligand recognition binding site, and γc is the signal transduction unit. IL-21R is mainly distributed in lymphatic tissues, including spleen, thymus, lymph nodes and peripheral blood lymphocytes, and can also be expressed in B, T cells and NK-derived cell lines. After IL-21 binds to its receptor IL-21R, it can activate Jak1 and Jak3 of the Jak family. Jak1 and Jak3 activate transcription factors STAT1 and STAT3, and weakly activate STAT4 and STAT5. In addition, IL-21 can also conduct signal transduction through phosphoinositide kinase (PI3-K) and mitogen-activated protein kinase (MAPK) pathways.

IL-21 can up-regulate the secretion of IFN-γ and other cytokines, induce apoptosis and exert tumor activity.
IL-21 can regulate NK cells.
IL-21 can promote the proliferation, differentiation and maturation of human bone marrow-derived NK cells, as well as enhances the cytotoxic activity of activated NK cells.
IL-21 can promote the growth of CD8+ T cells, proliferate and enhance the cytotoxic function of CD8+ T cells.
IL-21 can regulate dendritic cells.

1. Process samples.
2. IL-21 detection (qPCR, Enzyme-linked immunosorbent assay (ELISA), Flow cytometry).
3. Analysis results.

Sample Types-Blood, serum, plasma, cerebrospinal fluid, cell culture supernatant, tissue homogenate, cell culture medium, urine, tumor, etc.