IL-25 Detection (CAT#: STEM-MB-0292-WXH)

Introduction

Interleukin 25 (IL-25), also known as IL-17E, is a newly discovered member of the IL-17 cytokine family, but is only 16% homologous to IL-17A in amino acid sequence. IL-25 is mainly derived from mast cells, eosinophils and basophils stimulated by stem cell factors. In addition, intestinal epithelial cells and brain microvascular endothelial cells can also secrete IL-25. IL-25 can induce the differentiation of non-T cells, non-B cells, and hematopoietic stem cells (C-kit), thereby initiating an immune response and expelling intestinal parasites. IL-25 can characteristically induce eosinophil infiltration, activate Th2 cell response, and up-regulate the expression level of IL-4, IL-5, IL-13 and other cytokines. The production of these cytokines is also related to increase of serum IgE, IgGl, IgA level and the infiltration of eosinophils in various tissues.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

The biological activity of IL-25 is regulated by the transmembrane transport receptor IL-25R (also called IL-17RB). It is a single 56kD transmembrane protein, and its intracellular and extracellular regions are relatively conserved Cysteine sequence structure, whose gene is fixed at 3p21 of human chromosome, and has homology with IL-17R. Human IL-25R is highly expressed in T cells of kidney, liver and intestine, especially Th2 memory cells. In addition, cells in peripheral blood and mucosa can express IL-25R highly. IL-25 and IL-17RB combine to play a biological role, but in fact the receptor of IL-25 should be a heterodimer composed of IL-17RA and IL-17RB. Although IL-25 does not bind to IL-17RA, IL-17RA gene knockout cannot effectively stimulate the production of IL-5 and IL-13, indicating that both IL-17RA and IL-17RB are involved in IL-25 signaling. After IL-25 is combined with IL-25R, it can activate NF-κB to transmit signals by interacting with TRAF6.

Applications

IL-25 can promote the proliferation of lymphoid lineages.
IL-25 can characteristically induce eosinophil infiltration, activate Th2 cell response, and up-regulate the expression level of IL-4, IL-5, IL-13 and other cytokines.

Procedure

1. Process samples.
2. IL-25 detection (qPCR, Enzyme-linked immunosorbent assay (ELISA), Flow cytometry).
8. Analysis results.

Notes

Sample Types-Blood, serum, plasma, cerebrospinal fluid, cell culture supernatant, tissue homogenate, cell culture medium, urine, tumor, etc.

Other recommended products

BCR Pathway Assay (CAT#: STEM-MB-0344-WXH)
IL-11 Detection (CAT#: STEM-MB-0280-WXH)
CX3C Subfamily Detection Service (CAT#: STEM-MB-0262-WXH)
Death Receptor Pathway Assay (CAT#: STEM-MB-0346-WXH)
IL-34 Detection (CAT#: STEM-MB-0300-WXH)
IL-32 Detection (CAT#: STEM-MB-0298-WXH)
TNF Signaling Pathway Assay (CAT#: STEM-MB-0342-WXH)
IFN-γ Detection (CAT#: STEM-MB-0269-WXH)