IL-25 Detection (CAT#: STEM-MB-0292-WXH)

Introduction

Interleukin 25 (IL-25), also known as IL-17E, is a newly discovered member of the IL-17 cytokine family, but is only 16% homologous to IL-17A in amino acid sequence. IL-25 is mainly derived from mast cells, eosinophils and basophils stimulated by stem cell factors. In addition, intestinal epithelial cells and brain microvascular endothelial cells can also secrete IL-25. IL-25 can induce the differentiation of non-T cells, non-B cells, and hematopoietic stem cells (C-kit), thereby initiating an immune response and expelling intestinal parasites. IL-25 can characteristically induce eosinophil infiltration, activate Th2 cell response, and up-regulate the expression level of IL-4, IL-5, IL-13 and other cytokines. The production of these cytokines is also related to increase of serum IgE, IgGl, IgA level and the infiltration of eosinophils in various tissues.

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Principle Applications Procedure Materials Notes Related Products

Principle

The biological activity of IL-25 is regulated by the transmembrane transport receptor IL-25R (also called IL-17RB). It is a single 56kD transmembrane protein, and its intracellular and extracellular regions are relatively conserved Cysteine sequence structure, whose gene is fixed at 3p21 of human chromosome, and has homology with IL-17R. Human IL-25R is highly expressed in T cells of kidney, liver and intestine, especially Th2 memory cells. In addition, cells in peripheral blood and mucosa can express IL-25R highly. IL-25 and IL-17RB combine to play a biological role, but in fact the receptor of IL-25 should be a heterodimer composed of IL-17RA and IL-17RB. Although IL-25 does not bind to IL-17RA, IL-17RA gene knockout cannot effectively stimulate the production of IL-5 and IL-13, indicating that both IL-17RA and IL-17RB are involved in IL-25 signaling. After IL-25 is combined with IL-25R, it can activate NF-κB to transmit signals by interacting with TRAF6.

Applications

IL-25 can promote the proliferation of lymphoid lineages.
IL-25 can characteristically induce eosinophil infiltration, activate Th2 cell response, and up-regulate the expression level of IL-4, IL-5, IL-13 and other cytokines.

Procedure

1. Process samples.
2. IL-25 detection (qPCR, Enzyme-linked immunosorbent assay (ELISA), Flow cytometry).
8. Analysis results.

Notes

Sample Types-Blood, serum, plasma, cerebrospinal fluid, cell culture supernatant, tissue homogenate, cell culture medium, urine, tumor, etc.

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