IL-6 Detection (CAT#: STEM-MB-0275-WXH)

IL-6 and Interleukin-6 Receptor(IL-6R) combine to transmit signals to produce various biological effects. IL-6R can be divided into specific structural chains IL-6R and signaling chain gp130. With a molecular weight of 130kD, gp130 is a type of glycopeptide with transmembrane signaling function, which is expressed on the surface of most cells; IL-6R has a molecular weight of 80kDa, and its expression has been more restricted, mainly found in liver cells, Neutrophils, monocytes, and CD4+ T cells. Therefore, cells expressing gp130 but not IL-6R cannot bind to IL-6 to cause a signal response.
During signal transmission, IL-6 and IL-6R form the IL-6R / IL-6Rα complex, which then binds to IL-6Rβ, then activates the janus kinase and starts the enzymatic reaction. JAK can phosphorylate the cytoplasmic portion of gp130 internal tyrosine residues.And the most prominent of the factors are STAT3, STAT1, forming the classic JAK-STAT signaling pathway.

IL-6 can participate in regulating the immune response, and can directly participate in the local inflammatory response and the inflammatory damage process.
IL-6 can stimulate cell growth and promote cell differentiation, such as promoting T cell proliferation and stimulating cytotoxic T cell responses, promoting B cells activate proliferation and differentiation, and inducing stem cells to synthesize acute phase proteins.

1. Process samples.
2. IL-6 detection (qPCR, Enzyme-linked immunosorbent assay (ELISA), Flow cytometry).
3. Analysis results.

Sample Types-Blood, serum, plasma, cerebrospinal fluid, cell culture supernatant, tissue homogenate, cell culture medium, urine, tumor, etc.