Imaging of Extracellular Vesicles in Three Dimensions by Direct Stochastic Optical Reconstruction Microscopy (CAT#: STEM-MIT-0374-LJX)

Introduction

Extracellular vesicles are membranous vesicles released by cells into extracellular matrix. They are involved in cell communication, cell migration, angiogenesis and tumor cell growth. They exist widely in various body fluids and cell supernatants, and carry some important signaling molecules stably.
The visualization of extracellular vesicles often require direct stochastic optical reconstruction microscopy due to their small diameter (40-250 nm), which is beneath the diffraction limit of typical light microscopy.

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Principle Applications Procedure Materials Notes Related Products

Principle

Principles of stochastic optical reconstruction microscopy: By fitting the two-dimensional Gaussian function to determine the centroid of microscope-formed light spots, a single fluorescent source (such as a fluorescent group) can be located with high precision. The accuracy of the calculation to determine the centroid depends only on the number of photons collected, and the resolution scale can be tens of nanometers or smaller. To achieve this accuracy, the density of the fluorescent molecules being tested is required to be low enough that the spots of the two fluorescent groups are unlikely to overlap.

Applications

Imaging in two or three dimensions, in multiple colors, and even in living cells
Applied in many areas of the life sciences, and provides very high resolution images for many different needs from neuroscience to subcellular science

Procedure

1. Sampling
2. Preparation of slices
3. Staining (Select according to the specific experimental situation)
4. Observation

Materials

• Sample Type:
Extracellular Vesicles

Notes

Operate in strict accordance with the operating procedures, and shall not arbitrarily change the operating procedures

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