Imaging of Microtubule Arrays in Intact Arabidopsis thaliana Seedling Roots by Stochastic Optical Reconstruction Microscopy (CAT#: STEM-MIT-0379-LJX)

Introduction

Arabidopsis thaliana is a delicate annual herb of the genus Arabidopsis in the cruciferous family. Arabidopsis thaliana is a typical self - crossbreeding plant and has a leading position in plant genetic research. Arabidopsis thaliana grows in a wide geographical range and has various species, which is very conducive to the study of plant adaptation to the environment.
Super-resolution fluorescence microscopy has generated tremendous success in revealing detailed subcellular structures in animal cells. However, its application to plant cell biology remains extremely limited due to numerous technical challenges, including the generally high fluorescence background of plant cells and the presence of the cell wall. In the service, stochastic optical reconstruction microscopy (STORM) imaging of intact Arabidopsis thaliana seedling roots with a spatial resolution of 20-40 nm was demonstrated.

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Principle Applications Procedure Materials Notes Related Products

Principle

Principles of stochastic optical reconstruction microscopy: By fitting the two-dimensional Gaussian function to determine the centroid of microscope-formed light spots, a single fluorescent source (such as a fluorescent group) can be located with high precision. The accuracy of the calculation to determine the centroid depends only on the number of photons collected, and the resolution scale can be tens of nanometers or smaller. To achieve this accuracy, the density of the fluorescent molecules being tested is required to be low enough that the spots of the two fluorescent groups are unlikely to overlap.

Applications

Imaging in two or three dimensions, in multiple colors, and even in living cells
Applied in many areas of the life sciences, and provides very high resolution images for many different needs from neuroscience to subcellular science

Procedure

1. Sampling
2. Preparation of slices
3. Staining (Select according to the specific experimental situation)
4. Observation

Materials

• Sample Type:
Arabidopsis thaliana Seedling Roots

Notes

Operate in strict accordance with the operating procedures, and shall not arbitrarily change the operating procedures

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