Immobilized Avidin, 25ml Resin, 786-594, G-BIOSCIENCES (CAT#: STEM-C-3122-LGZ)

Biotin is a 244Da vitamin (vitamin H) molecule that exhibits an extraordinary binding affinity for avidin (Ka=1015 M-1). The biotin and avidin interaction is fast and once the bond is established it can survive up to 3M guanidine-HCl and extreme pH. The biotin-avidin bond can only be reversed by denaturing the avidin protein molecule with 8M guanidine-HCl at pH 1.5 or by boiling in SDS Page Sample Loading Buffer.

Avidin is a glycoprotein that derives approximately 10% of its total mass from carbohydrates. Avidin has a molecular weight of 67 kDa and consists of four identical 128 amino acid subunits, each of which has a biotin-binding domain. Avidin is a basic protein with an isoelectric pH of 10-10.5 and readily soluble in aqueous buffers containing various salts, pH (2-11), temperature and other laboratory reagents. This broad tolerance makes avidin suitable for a wide variety of analytical applications. Avidin has an extraordinary binding affinity for biotin (Ka=1015M-1).

Avidin is covalently coupled to agarose resulting in minimal leaching and is stable above pH 2-11.

Avidin resins are designed for single-step small and large-scale affinity purification of proteins and antibodies with biotin tags. This resin can also be used for immunoprecipitation using biotinylated antibodies. Provide 50% resin paste.

Specific binding and elution buffers are also available.

Size: 25ml resin

Avidin covalently coupled to ~6% cross linked agarose. Minimal Leaching
Binding capacity 15-20µg biotin/ml resin
Bead Structure: 6% cross-linked agarose

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