Investigation of Polyethylenimine (PEI) intracellular transport by Nanoparticle tracking analysis (NTA) (CAT#: STEM-MB-0741-WXH)

Introduction

Polyethylenimine (PEI) is a simple, inexpensive and effective reagent for condensing and linking plasmid DNA to adenovirus for gene delivery. PEI has a number of uses in laboratory biology, especially tissue culture, but is also toxic to cells if used in excess. Toxicity is by two different mechanisms, the disruption of the cell membrane leading to necrotic cell death (immediate) and disruption of the mitochondrial membrane after internalisation leading to apoptosis (delayed).