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Investigation of the effect of sulfomethoxypyrazine-pyrimethamine therapy on the fine structure of Toxoplasma gondii-cysts in the brain of Mastomys natalensis by scanning and transmission electron microscope technology (CAT#: STEM-MIT-0076-LJX)

Introduction

Two month old chronically infected Mastomys natalensis, which had been treated for ten days with sulfomethoxypyrazine-pyrimethamine, were utilized for testing the effect of chemotherapy on the fine structure of Toxoplasma cysts (strain ALT). After completion of the therapy, cysts were examined from the brains of killed animals in scanning and transmission electron microscopes.




Principle

Scanning electron microscope (SEM) is another tool to study the surface morphology, which is different from transmission electron microscope and optical microscope. SEM uses extremely narrow electron beams to scan the sample and uses point-by-point imaging to obtain an enlarged image. SEM generates secondary electron emission through the interaction between the electron beam and the sample, and the secondary electron can produce the morphologic image of the sample surface enlargement. SEM can directly utilize the material properties of the sample surface for microscopic imaging.
SEM provides the possibility to study the relationship between the three-dimensional structure of cell or tissue surface and antigen composition. The markers used in scanning electron microscopy should be able to be in the range of scanning electron microscopy, and have good localization ability to cell or tissue antigen. The selection of markers should be based on the purpose of the study. If the volume of the marker cells is large, large markers should be used, while small, easily identifiable markers should be selected to locate the receptor.

Applications

Imaging and analysis in the fields of biology, medicine, materials and chemistry

Procedure

1. Sampling
2. Preparation of slices
3. Staining (Select according to the specific experimental situation)
4. Observation

Materials

• Sample Type:
The brain of Mastomys natalensis

Notes

Turn off the power when the device is not in use
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