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LATE (Linear-After-The-Exponential) PCR (CAT#: STEM-MB-0209-WXH)

Introduction

Linear-After-The-Exponential (LATE)–PCR describes a new paradigm for primer design that renders assays as efficient as symmetric PCR assays, regardless of primer ratio. LATE-PCR generates single-stranded products with predictable kinetics for many cycles beyond the exponential phase. LATE-PCR also introduces new probe design criteria that uncouple hybridization probe detection from primer annealing and extension, increase probe reliability, improve allele discrimination, and increase signal strength by 80–250% relative to symmetric PCR. These improvements in PCR are particularly useful for real-time quantitative analysis of target numbers in small samples. LATE-PCR is adaptable to high throughput applications in fields such as clinical diagnostics, biodefense, forensics, and DNA sequencing.




Applications

• DNA sequencing and hybridization studies.
• Site-directed mutagenesis studies.
• Discrimination and identifying various alleles.

Procedure

General procedure:
1. Denaturation
2. Annealing
3. Extension
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