Lipofection (CAT#: STEM-GT-0002-WXH)

Introduction

Lipofection is commonly used to transfer nucleic acids such as RNA or DNA into eukaryotic cells. After protocol optimization, this method is easy to apply and yields highly reproducible results of transient and stable transfections.However, lipofection efficiency strongly depends on the cell type and has to be tested and optimized in advance.Especially for primary and non-dividing cells,the viability after the transfection process might be decreased due to the high cellular sensitivity. In contrast to membrane fusion,lipofection is based on endosomal molecule uptake,which might delay the time of analysis.

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Principle Applications Procedure Materials Notes Related Products

Principle

Usually, a mixture of neutral and cationic liposomes forms complexes with the nucleotides of interest. These complexes pass the cell membrane and then release the nucleotides into the cytoplasm via endocytosis. Then, the nucleotides either successfully escape the endosome or undergo lysosomal degradation, of which the latter might lead to reduced transfection efficiency. After endosomal escape, the nucleotide is expressed in the target cells.

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