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lncRNA Microarray (CAT#: STEM-MB-0147-WXH)

Introduction

Long non-coding RNA (IncRNA) refers to RNA molecules between 200-100000 nt in length, which do not encode proteins but are involved in the regulation of various processes in cells. Recent studies have shown that IncRNAs are involved in a variety of important regulatory processes such as X chromosome silencing, genomic imprinting and chromatin modification, transcriptional activation, transcriptional interference, and intranuclear transport, and are closely related to the occurrence, development and prevention of human diseases.
Traditional IncRNA screening methods are inefficient and have a high false positive rate. In contrast, gene microarrays have comprehensive coverage, high efficiency and accuracy, so using gene microarrays to screen lncRNAs is an accurate and fast method.




Applications

• Screening for differentially expressed lncRNAs and mRNAs
• lncRNA functional annotation
• Prediction of lncRNA regulatory mechanisms
• Molecular mechanism study of pathogenic gene expression

Procedure

1. Total RNA extraction.
2. Reverse transcription synthesis of cDNA.
3. In vitro transcription synthesis of CRNA.
4. Random primer synthesis of righteous strand cDNA.
5. DNA fragmentation, biotin labeling.
6. Chip hybridization, washing, staining and scanning.

Notes

Customer provides cells, fresh tissue samples or total RNA samples.

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