Mapping images of individual cells by Raman Spectroscopy (CAT#: STEM-ST-0036-WXH)

Introduction

Macroscopic resolution allows examination of the chemistry of individual cells by mapping their images. These images can contain full spectral information at each pixel so that the distribution of components within the cell can be visualized based on their Raman signature. This is extremely valuable to researchers as biochemical changes can be observed during a cell’s life cycle or when a cell is damaged or cancerous. Using confocal Raman microscopy, the changes in a variety of cells, including bacteria and eukaryotes can be monitored over time and comparison between healthy and diseased tissue states can be done easily.

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Principle Applications Procedure Materials Notes Related Products

Principle

Raman Spectroscopy is a non-destructive chemical analysis technique which provides detailed information about chemical structure, phase and polymorphy, crystallinity and molecular interactions.
The principle behind Raman spectroscopy is that the monochromatic radiation is passed through the sample such that the radiation may get reflected, absorbed, or scattered. The scattered photons have a different frequency from the incident photon as the vibration and rotational property vary.

Applications

• Analysis of biocompatibility of a material.
• Analysis of nucleic acids.
• Study of interactions between drugs and cells.
• Photodynamic therapy (PDT).
• Analyzing metabolic accumulations of a substance or compounds.
• Diagnosis of disease.
• Analysis of individual cells.
• Cell sorting applications.
• Analyzing the features of biomolecules.
• Study of bone structure.

Procedure

1. Preparation of samples
2. Determine instrument parameters
3. Perform background scan
4. Test the sample
5. Data analysis

Materials

• Raman Spectrometer
• Raman Imaging Microscope

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