Mass Cytometry (CAT#: STEM-CBT-0052-WXH)

Mass cytometry is a mass spectrometry technique based on inductively coupled plasma mass spectrometry and time of flight mass spectrometry used for the determination of the properties of cells (cytometry). In this approach, antibodies are conjugated with isotopically pure elements , and these antibodies are used to label cellular proteins. Cells are nebulized and sent through an argon plasma, which ionizes the metal-conjugated antibodies. The metal signals are then analyzed by a time-of-flight mass spectrometer. The approach overcomes limitations of spectral overlap in by utilizing discrete isotopes as a reporter system instead of traditional fluorophores which have broad emission spectra.

• Phenotypic characterization
• Intracellular cytokine assay
• Intracellular signaling state characterization
• Cell volume and size measurement
• Cell viability identification
• Cell cycle identification
• Proliferation tracing
• Receptor occupancy assay
• Tetramer-based antigen-specific T cell screening
• Chromatin modification analysis
• RNA and protein co-detection

1. Metal-labeled antibodies are used to recognize antigens on the cell surface or intracellularly
2. Cells are atomized and then ionized
3. The ion cloud enters the time-of-flight mass analyzer and records the arrival time of various metal ions
4. Detect the content of various labeled metals in the cells and generate mass spectrometry data
5. Data processing

Metallo-interchelators: the rhodium(Rh)-interchelator and iridium (Ir)-interchelator.
Metaltagged antibodies: antibodies are primarily tagged with the lanthanide series of metals