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Methylated DNA Binding Domain-Sequencing (MBD-Seq) is the use of proteins that specifically bind to highly methylated DNA to enrich highly methylated DNA, and then use high-throughput sequencing technology to sequence the enriched DNA method. To detect methylation sites on a genome-wide scale. This technology can specifically detect methylated modified regions in the genome, and is suitable for studies on genetic imprinting, embryonic development, and tumor development.