Molecular mass analysis of Monoclonal Antibodies by Static light scattering (SLS) (CAT#: STEM-MB-0571-WXH)

Introduction

A monoclonal antibody (mAb, more rarely called moAb) is an antibody produced from a cell lineage made by cloning a unique white blood cell. All subsequent antibodies derived this way trace back to a unique parent cell.
Monoclonal antibodies are being used in the diagnosis and treatment of many diseases, including some types of cancer. They can be used alone or to carry drugs, toxins, or radioactive substances directly to cancer cells.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Static light scattering is a technique in physical chemistry that measures the intensity of the scattered light to obtain the average molecular weight Mw of a macromolecule like a polymer or a protein in solution. Measurement of the scattering intensity at many angles allows calculation of the root mean square radius, also called the radius of gyration Rg. By measuring the scattering intensity for many samples of various concentrations, the second virial coefficient, A2, can be calculated.

Applications

The main applications of static light scattering is molecular mass determination of macromolecules, such as proteins and polymers, as it is possible to measure the molecular mass of proteins without any assumption about their shape.

Procedure

1. Sample preparation
2. Measurement by SLS instrument
3. Data analysis

Materials

• Right-Angle Light Scattering (RALS) Detector
• Low-Angle Light Scattering (LALS) Detector
• Hybrid RALS/LALS Detector
• Multi-Angle Light Scattering (MALS) Detector

Other recommended products

Molecular mass analysis of plasmid DNA by Static light scattering (SLS) (CAT#: STEM-MB-0570-WXH)
Characterization of antibody-polyol interactions by static light scattering by Static light scattering (SLS) (CAT#: STEM-MB-0583-WXH)
Molecular mass analysis of Carrageenans by Static light scattering (SLS) (CAT#: STEM-MB-0561-WXH)
Analysis of Lipopolysaccharide Aggregation by Static light scattering (SLS) (CAT#: STEM-MB-0588-WXH)
Molecular mass analysis of amylose by Static light scattering (SLS) (CAT#: STEM-MB-0587-WXH)
Molecular mass analysis of insulin self-association by Static light scattering (SLS) (CAT#: STEM-MB-0567-WXH)
Molecular mass determination of proteins by Static light scattering (SLS) (CAT#: STEM-MB-0559-WXH)
Molecular mass analysis of Cyclodextrins by Static light scattering (SLS) (CAT#: STEM-MB-0566-WXH)