Inquiry Basket

Molecular mass analysis of tobacco mosaic virus by Static light scattering (SLS) (CAT#: STEM-MB-0576-WXH)

Introduction

Tobacco mosaic virus causes a mottled browning of tobacco leaves, and accordingly is of major economic importance. It also infects other crops, most notably tomatoes. The virus is spread mechanically from infected plants to scratched or damaged leaves of normal plants.

Add to Cart Please Inquire

Principle Applications Procedure Materials

Principle

Static light scattering is a technique in physical chemistry that measures the intensity of the scattered light to obtain the average molecular weight Mw of a macromolecule like a polymer or a protein in solution. Measurement of the scattering intensity at many angles allows calculation of the root mean square radius, also called the radius of gyration Rg. By measuring the scattering intensity for many samples of various concentrations, the second virial coefficient, A2, can be calculated.

Applications

The main applications of static light scattering is molecular mass determination of macromolecules, such as proteins and polymers, as it is possible to measure the molecular mass of proteins without any assumption about their shape.

Procedure

1. Sample preparation
2. Measurement by SLS instrument
3. Data analysis

Materials

• Right-Angle Light Scattering (RALS) Detector
• Low-Angle Light Scattering (LALS) Detector
• Hybrid RALS/LALS Detector
• Multi-Angle Light Scattering (MALS) Detector

Other recommended products

Molecular mass analysis of amylose by Static light scattering (SLS) (CAT#: STEM-MB-0587-WXH)
Molecular mass analysis of Soybean polysaccharide (SPS) by Static light scattering (SLS) (CAT#: STEM-MB-0562-WXH)
Molecular mass analysis of Carrageenans by Static light scattering (SLS) (CAT#: STEM-MB-0561-WXH)
Molecular mass analysis of OmpF porin by Static light scattering (SLS) (CAT#: STEM-MB-0572-WXH)
Molecular mass analysis of insulin self-association by Static light scattering (SLS) (CAT#: STEM-MB-0567-WXH)
Molecular mass analysis of Monoclonal Antibodies by Static light scattering (SLS) (CAT#: STEM-MB-0571-WXH)
Molecular mass analysis of protein-protein interactions by Static light scattering (SLS) (CAT#: STEM-MB-0564-WXH)
Molecular mass analysis of aggregation of jack bean urease by Static light scattering (SLS) (CAT#: STEM-MB-0575-WXH)

We use cookies to understand how you use our site and to improve the overall user experience. This includes personalizing content and advertising. Read our Privacy Policy

Accept Cookies