Monitoring the pH change of plant tissue medium by chlorophenol red (CAT#: STEM-PPA-0037-LJX)

Introduction

The use of pH indicators provides a simple, semi-quantitative visual method for quickly assessing pH changes in tissue culture media. In this service, chlorophenol red, bromocresol purple, and bromocresol green were tested to assess their functionality in the growth medium for plant shoot cultures. All the pH indicators showed no toxic side effects for the plant species tested in this study and were able to be autoclaved to ensure media sterility. The addition of these pH indicators to quickly assess media pH in large tissue culture collections can aid in routine maintenance.

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Principle Applications Procedure Materials Notes Related Products

Principle

Acid-base indicators produce different colors in aqueous solutions of their specific pH range. The indicator is added to the standard buffer solution, and the resulting color is used as the standard colorimetric tube. The pH value of the water sample can be measured by visual colorimetric comparison with the water sample colorimetric tube with the same indicator.

Applications

For determining the approximate PH value of solutions in food production, wastewater treatment, pharmaceutical and chemical industries

Procedure

1. Add ph indicator to the standard solution, and the solution with different pH will show different colors. Use these solutions as standard colorimetric tubes
2. Add the same ph indicator to the solution to be tested, and compare the color of the solution to be tested with the standard colorimetric tube to determine the approximate ph value of the solution

Materials

• Sample Type:
Plant tissue medium

Notes

After dropping the indicator into the solution, it should be gently shaken to avoid inaccurate detection.

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