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Monoclonal Antibody Production (CAT#: STEM-MB-0116-WXH)

Introduction

Monoclonal antibodies are highly homogeneous antibodies produced by a single B cell clone that only target a specific antigenic epitope. It is usually prepared by hybridoma technology. Hybridoma antibody technology is based on cell fusion technology, which combines sensitized B cells with the ability to secrete specific antibodies and myeloma cells with unlimited reproductive capacity into B cell hybridoma.
Using a single hybridoma cell with this characteristic to cultivate a cell population, a specific antibody against an antigenic epitope can be prepared, that is, a monoclonal antibody. Because it only acts on a certain antigenic determinant on the antigen complex molecule, it has the characteristics of strong specificity and high potency.




Applications

• Used to probe the fine structure of proteins Neoantigens on the surface of subfrequent lymphocytes; histocompatibility antigens
• Radioimmunoassays for Hormones and Drugs
• Tumor localization and classification
• Purification of microbial and parasite antigens
• Immunotherapy and drug-combined immuno-chemotherapy

Procedure

1. Antigen Preparation
2. Animal immunization
3. Hybridoma cells
4. Proliferation and Screening
5. Antibody Purification
6. Potency detection
7. Antibody modification

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