Unlock Exclusive Discounts & Flash Sales! Click Here to Join the Deals on Every Wednesday!

Morphological study of special extracellular matrix within the renal stem/progenitor cell niche by transmission electron microscopy technology (CAT#: STEM-MIT-0021-LJX)

Introduction

Stem cell is a kind of multi-potential cell which has the ability of self-renewing. Stem cells can be divided into embryonic stem cells and adult stem cells. According to different differentiation potentials, they can be divided into topotent stem cells, pluripotent stem cells, and unipotent stem cells, which have the characteristics of multidirectional differentiation potential, infinite division, proliferation and so on.




Principle

Transmission electron microscopy (TEM) is to project the accelerated and concentrated electron beam onto a very thin sample, and the electron collides with the atoms in the sample and changes the direction, thus generating the stereo scattering Angle. The size of the scattering Angle is related to the density and thickness of the sample, so the image can be formed with different shades. The image can be enlarged, focused and displayed on imaging devices such as fluorescent screens, film and photosensitive coupling components. The resolution of transmission electron microscope is much higher than that of optical microscope, can reach 0.1~0.3nm, magnification of tens of thousands to millions of times. Therefore, transmission electron microscopy can be used to observe the fine structure of the sample.

Applications

Microscopic imaging in materials science or biology.

Procedure

1. Sampling
2. Preparation of slices
3. Staining (Select according to the specific experimental situation)
4. Observation

Materials

• Sample Type:
Renal stem/progenitor cell

Notes

Pay attention to air humidity
Voltage needs to be stabilized
Advertisement