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Morphological study of thin sections of drosophila by transmission electron microscopy technology (CAT#: STEM-MIT-0010-LJX)

Introduction

Fruit flies are members of the Drosophila family diptera.
Some species of fruit fly live on rotting fruit or in fungi or fleshy flowers. Its body size is small, mostly yellow brown. The head has a pair of large and bright red compound eyes, two compound eyes have three pairs of eye margin bristles on the inside, and the center of the back head between the compound eyes is slightly raised to form a monocular triangle area, and the front and lower sides of the three monocular have a pair of monocular bristles. Distributed all over the world and wintered in human homes.




Principle

Transmission electron microscopy (TEM) is to project the accelerated and concentrated electron beam onto a very thin sample, and the electron collides with the atoms in the sample and changes the direction, thus generating the stereo scattering Angle. The size of the scattering Angle is related to the density and thickness of the sample, so the image can be formed with different shades. The image can be enlarged, focused and displayed on imaging devices such as fluorescent screens, film and photosensitive coupling components. The resolution of transmission electron microscope is much higher than that of optical microscope, can reach 0.1~0.3nm, magnification of tens of thousands to millions of times. Therefore, transmission electron microscopy can be used to observe the fine structure of the sample.

Applications

Microscopic imaging in materials science or biology.

Procedure

1. Sampling
2. Preparation of slices
3. Staining (Select according to the specific experimental situation)
4. Observation

Materials

• Sample Type:
Thin sections of drosophila

Notes

Pay attention to air humidity
Voltage needs to be stabilized
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