MWCO Ultrafiltration Spin Units, 12 mL, Pack Of 24, MWCO 50 K, 17-0291-50, Marvelgent biosciences (CAT#: STEM-GT-3055-LGZ)

Ultrafiltration spin devices provide a simple one-step procedure for desalting and concentrating proteins and other macromolecules with recoveries greater than 90%. The spinning device adopts a molecular weight cut-off polyethersulfone (PES) filter membrane, which can intercept macromolecules with a large molecular weight and filter molecules such as salts with a small molecular weight. Due to the low protein/DNA binding properties of the PES filter, loss of retentate on the filter is minimized. High retentate recovery.

Four molecular weight cut-off (MWCO) sizes are available to cover a broad molecular weight range: 10K, 30K, 50K and 100K. It is recommended to choose a MWCO that is 1/2 ~ 1/3 smaller than the protein to be concentrated. For example, to concentrate an 80 kDa protein, we recommend starting with a 30 K MWCO spin unit.

MWCO: 50 K
Maximum sample capacity: 12 mL with a swinging-bucket rotor; 10 mL with a Fixed-angle rotor (Min. rotor angle is 25°)
Material of construction: Screw cap: High-density Polyethylene (HDPE); Sealing gasket: Polyethylene (PE); Filtrate receiver tube: Polypropylene (PP); Filter unit: Methyl methacrylate-Butadiene-Styrene (MBS); Ultrafiltration membrane: Polyethersulfone (PES)
Active ultrafiltration membrane area: 5.48 cm2
Product dimension: Assembled unit height: 119 mm; Assembled unit O.D.: 29.3 mm; Filter unit height: 72.0 mm; Filter unit O.D. : 29.5 mm
Wetted part: No
DNase/RNase: No detectable DNase/RNase contamination
Maxi. centrifugation speed: Swinging-bucket: 4,000xg; Fixed angle: 5,000xg
Working temperature: -20°C to 50°C
Chemical stability: Stable in most commonly used reagents for protein and nucleic acid applications
Chemical incompatibility: Acetic Acid (≥ 25%); Acetonitrile (≥ 30%); Aliphatic & aromatic esters; Amines; Ammonium hydroxide (≥ 5%); Aromatic & chlorinated hydrocarbons; Butyl acetate (≥ 40%); Dimethyl acetamide (DMAC) (≥ 30%); Ethers; Hydrochloric acid (≥ 0.5 N at 50°C); Isopropyl Alcohol (≥ 25%); Ketone; Methylene chloride (≥ 1%); Methyl ethyl ketone (≥ 1%); Phosphoric acid (≥ 1 N); Sodium deoxycholate (≥ 5%); Sodium hydroxide (≥ 0.5N at 50°C); Sodium hypochlorite (≥ 0.04%); Tetrahydrofuran (≥ 5%); Toluene (≥ 1%)
Sterility: Non-sterile
Autoclavability: No
Quality assurance: Manufactured in accordance with ISO 13485 : 2016 & ISO 9001 : 2015 quality management systems.
Package size: 24 spin units per pack.
Storage: Room temperature. Keep dry.
Application note: Single use only.

Simple, fast spin, simultaneous desalting and concentration (up to 75X concentration).
Buffer exchange in two quick steps.
Faster Filtration - High throughput PES membrane heat sealed to housing unit.
High Retentate Recovery - PES membranes have very low protein and DNA/RNA absorption properties and the filter housing unit is also constructed of low binding materials.
Feasibility of collecting filtrate – the choice of retaining the retentate or retaining the filtrate is flexible.
A dead stop bag is built into the bottom of the filter unit, so there is no risk of drying out during spinning.
Vertical position of filter membrane - reduces clogging/fouling problems.
Less processing time, less buffer, less mess than dialysis.
Flexible throughput - it's easy to execute multiple samples in parallel or scale up with the same sample.
No DNase/RNase contamination detected.

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