Compared with the traditional N-STORM, the N-STORM 4.0 Super-Resolution Microscope's acquisition speed is ten times faster, which can achieve live cell imaging with nanometer resolution. N-STORM 4.0 uses an improved laser excitation design and sCMOS camera to increase the acquisition speed from a few minutes to a few seconds, while collecting an incredible number of polymers. In addition, the system provides greater flexibility in the imaging field of view, allowing users to choose the mode most suitable for their application, whether it is high-speed imaging of live cells or large field of view collection of fixed cells.