NanoGel dT20 mRNA Affinity Resin, NanoGel dT20 10mL (CAT#: STEM-C-3248-ZJF)

NanoGel dT20 is specifically engineered to meet the selectivity and capacity demands of large-scale downstream purification for various mRNA constructs used in vaccine and gene therapy applications. This resin efficiently captures mRNA through its polyadenylated (polyA) tail using straightforward purification steps involving salt for binding and water for elution. NanoGel dT20 ensures effective separation of mRNA from transcription reaction components, including enzymes and plasmid DNA. It provides a robust and efficient platform for the purification of diverse mRNA molecules across a range of applications.

Chromatography Technique: mRNA affinity
Matrix: PS/DVB
Particle Size: 50 µm
Ligand: dT-20mer
Dynamic Binding Capacity: ~2 mg·mL⁻¹ (mRNA)
Maximum Pressure: 3 MPa
Clean In Place: 0.1 M NaOH
Recommended Flow Velocity: 50-300 cm/h
pH Stability: 2-13
Chemical Stability: Stable in commonly used buffers, 2 M HAc, 1 M HCl, 100% ethanol, isopropyl alcohol, etc. Avoid long-term exposure to strong oxidant, benzyl alcohol, or tetrahydrofuran.
Operational Temperature: 2-65 ℃
Storage: 20% Ethanol, 2-8 ℃

Monodisperse matrix of very wide pores provides high binding capacity
Simplified workflow, high efficiency
Excellent scalability

STEM-C-3246-ZJF
STEM-C-3247-ZJF
STEM-C-3249-ZJF
STEM-C-3250-ZJF