The manufacturer of the Nexera UHPLC System, Shimadzu is Shimadzu. Refurbished Shimadzu Nexera UHPLC complete with Nexera SIL-30AC Autosampler, (2) Nexera LC-30AD pumps, DGU-20A5 Prominence Degasser (p/n 228-45019-32), & CBM-20A controller. LC-30AD: This parallel double-plunger model provides superior performance at micro flow rates, and enables stable delivery over a wide range of flow rates, from 0.0001 to 3.0000 mL/min (with a maximum operating pressure of 130 MPa) or from 3.001 to 5.000 mL/min (with a maximum operating pressure of 80 MPa). Its ultrahigh-pressure capacity allows using long columns packed with micro particles. It smoothly tracks settings and provides precise solvent delivery even for programs with sudden changes in concentrations, which is desirable for ultrafast gradient chromatography. SIL-30AC: The total-volume injection SIL-30AC autosampler features a pressure tolerance of 130 MPa as well as the world’s fastest sample injection (10 seconds), which dramatically reduces the total cycle time. It includes auto pretreatment and overlapping functions as standard, and an optional loop-injection method configuration to minimize delay volume. With reduction of the needle contact area, special coatings, surface treatments, and a new needle seal, the SIL-30AC reaches a new level of low carryover performance, which is especially beneficial for LCMSMS analysis. In addition, the included sample cooler features a dehumidifier function for storing samples at a constant temperature between 4°C and 40°C.