Ni-NTA Resin, 1ml Spin Column, 5 Columns, 786-944, G-BIOSCIENCES (CAT#: STEM-C-3198-LGZ)

Ni-NTA resin is a high-binding, high-capacity nickel-containing IMAC resin that can purify recombinant proteins with polyhistidine (6XHis) sequences. The resin is 6% cross-linked agarose with complete structure, the Ni2+/ml resin capacity is 20-40μmol, and the protein binding capacity is 50mg/ml. In fact, we have demonstrated the binding of 100mg/ml resin to a 50kDa 6XHis-tagged protein. Available as ready-to-use resins in 5 convenient sizes (10, 25, 100, 500 and 2 x 500ml) or supplied prepackaged in various spin column formats (0.2, 1 and 3ml) and spin plates.

Immobilized metal ion affinity chromatography (IMAC) was developed by Porath (1975) and is based on the association of certain protein residues (histidine, cysteine and to some extent tryptophan) with transition metals cation interactions. Ni-NTA resin is designed for the purification of recombinant proteins fused to polyhistidine (6XHis) tags expressed in bacterial, insect and mammalian cells. The resin has high affinity and selectivity for recombinant fusion proteins tagged with 6 tandem histidine residues. Ni-NTA resin can be used to purify 6X His-tagged proteins under native and denaturing conditions. Resin-bound proteins can be competitively eluted with low pH buffers or with imidazole or histidine. Ni-NTA resin uses nitric acid triacetic acid (NTA), a tetra-acid chelating ligand, in a highly cross-linked 6% agarose matrix. NTA binds Ni2+ ions through four coordinations.

The resin bed volumes of the spin columns were 0.2, 1 and 3 ml, respectively, and the total column volumes were 1, 8 and 22 ml, respectively. The column is available as a spin format for gravity flow columns. Can also process up to 96 samples in a 96-well spin plate format.

Immobilized IDA nickel, copper, cobalt and zinc chelate resins and cobalt NTA resins are also available. Cobalt has the highest selectivity, followed by zinc, nickel and copper, but has the lowest binding capacity. Copper has the highest binding strength, followed by nickel and then zinc.

Specific binding/wash and elution buffers are available.

Our HOOK™ 6X His Protein Purification Kit includes everything needed to purify 6X His-tagged proteins from yeast or bacteria, including lysis buffer, lyase, resin, column and binding, washing and elution buffers. Each kit is available with nickel or cobalt chelate resins. Available kits are:

HOOK™ 6X His Protein Purification (Bacteria): Isolated from bacteria; optimized to produce up to 10mg/250ml of soluble 6X His-tagged protein.
HOOK™ 6X His Protein Spin Purification (Bacteria): Isolation from bacteria; optimized to produce ~1mg/50ml of soluble 6X His-tagged protein culture
HOOK™ 6X His Protein Purification (Yeast): Isolated from yeast; optimized to yield up to 10 mg of soluble 6X His-tagged protein.
HOOK™ 6X His Protein Spin Purification (Yeast): Isolated from yeast; optimized to yield up to 1 mg of soluble 6X His-tagged protein.

Size: 5 columns
High capacity: >50mg/ml
Ligand density: 20-40μmoles Ni2+ /ml resin
Bead Structure: 6% cross-linked agarose
Uses nitrilotriacetic acid (NTA), a tetradenate chelating ligand

G-Sep™ Ni NTA Agarose Fast Flow, 500ml, 786-1043, G-BIOSCIENCES (CAT#-STEM-C-3178-LGZ)
G-Trap™ Ni IDA Agarose Fast Flow, 1 ml, 5 Columns, 786-1021, G-BIOSCIENCES (CAT#-STEM-C-3179-LGZ)
G-Trap™ Ni IDA Agarose Fast Flow, 5 ml, 5 Columns, 786-1022, G-BIOSCIENCES (CAT#-STEM-C-3180-LGZ)
His Binding/ Wash Buffer (10mM Imidazole, 300mM NaCl, 50mM Sodium Phosphate (pH8.0)), 100ml, 786-542, G-BIOSCIENCES (CAT#-STEM-C-3181-LGZ)
His Elution Buffer (250mM Imidazole, 300mM NaCl, 50mM Sodium Phosphate (pH8.0)), 100ml, 786-543, G-BIOSCIENCES (CAT#-STEM-C-3182-LGZ)
Ni-NTA Magnetic Beads, 1ml, 786-910, G-BIOSCIENCES (CAT#-STEM-C-3183-LGZ)