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NmLOAD 26/500 (CAT#: STEM-C-3835-ZJF)

Highlights

Variety of SEC Columns: Prepacked with agarose-based SEC resins (NW Super 75/100/150/200/300) or dextran-based SEC resins (NW Dex G-10/15/25/50/75/100).
Flexible Column Sizes: Short column bed heights (20mm to 300mm) for desalting and longer column bed heights (100mm to 1000mm) for fractionation of proteins and polymers based on molecular size.
Prepacked Columns for Different Volumes: Available in sizes ranging from 1mL to liters for both short bed height (NmTRAP/NmSCREEN) and long bed height (NmVALID/NmLOAD) columns.

Cat Number: STEM-C-3835-ZJF

Application: For the purification, separation, and analysis of biomolecules, including proteins, nucleic acids, antibodies, and other macromolecules, across research and industrial applications.

Model: NmLOAD 26/500

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Description

Various SEC (size exclusion chromatography) columns are available, prepacked with agarose-based SEC resins such as NW Super 75/100/150/200/300 or dextran-based SEC resins like NW Dex G-10/15/25/50/75/100. These columns are designed with varying sizes and hardware materials. Short column bed heights (20–300 mm) are commonly used for group separation, particularly desalting, while longer bed heights (100–600 mm or even up to 1000 mm) are suitable for fractionating proteins and polymers with varying molecular weights or sizes. Short bed height columns, ranging from 1 mL to several liters, include options like NmTRAP, NmSCREEN, NmPREP, NmVALID, NmLOAD, and NmPILOT. Longer bed height columns, ranging from 20 mL to 750 mL, are also available for advanced applications.
For hydrophobic interaction chromatography (HIC), a variety of columns are prepacked with polymer-based HIC resin families such as NanoHR (PS-DVB), UniHR (PMMA), and Uni (PMMA), or sugar-based resins like NW Rose (agarose) and NW Rose Plus (agarose). These resins include frequently used functional groups like Phenyl, Octyl, Butyl, and Ether. HIC columns vary in size and hardware materials, with small columns (1–5 mL) designed for early-stage media screening, medium columns (10–50 mL) for method development and optimization, larger columns (100–500 mL) for method characterization and validation, and prepacked pilot-scale columns (2–10 L) for larger purification and product preparation needs.
Ion exchange chromatography (IEX) columns are offered with prepacked polymer-based resins such as NanoGel (PS-DVB), Nano (PS-DVB), UniGel (PMMA), and Uni (PMMA), as well as sugar-based resins like NW Rose (agarose), NW Rose Plus (agarose), and NW Dex (dextran). These IEX resins include functional groups such as SCX (SP), SAX (Q), WCX (CM), and WAX (DEAE), catering to a wide range of purification applications.
Affinity chromatography columns are also available, prepacked with media such as Protein A/G/L resins (UniMab50, UniMab50 HC, NMab, NMab Pro), IMAC affinity resins (UniIDA, UniNTA, NW Rose Ni FF), and other specific affinity resins (NM90 Agarose Blue, NW Rose GST 4FF). These columns are optimized for the purification of various biomolecules in scientific, diagnostic, and therapeutic applications.

Specification

Column Bed ID: 26 mm
Column Bed Height: 500 mm
Volume: 265 mL
Column Hardware Materials: PP
Max Pressure: 1.5 MPa

Features

Variety of SEC Columns: Prepacked with agarose-based SEC resins (NW Super 75/100/150/200/300) or dextran-based SEC resins (NW Dex G-10/15/25/50/75/100).
Flexible Column Sizes: Short column bed heights (20mm to 300mm) for desalting and longer column bed heights (100mm to 1000mm) for fractionation of proteins and polymers based on molecular size.
Prepacked Columns for Different Volumes: Available in sizes ranging from 1mL to liters for both short bed height (NmTRAP/NmSCREEN) and long bed height (NmVALID/NmLOAD) columns.
Diverse HIC Resin Families: Includes polymer-based HIC resins (NanoHR, UniHR, Uni) and sugar-based HIC resins (NW Rose, NW Rose Plus), with functional groups like Phenyl, Octyl, Butyl, and Ether.
HIC Column Sizes for Various Applications: Columns range from 1mL (NmTRAP/NmSCREEN) for early-stage screening to 10L (NmPILOT) for pilot scale purification.
Ion Exchange Columns for Various Applications: Prepacked with polymer-based resins (NanoGel, Nano, UniGel) or sugar-based resins (NW Rose, NW Dex), available in SCX, SAX, WCX, and WAX functional groups.
Affinity Columns for Specific Applications: Includes Protein A/G/L media (UniMab50/UniMab50 HC), IMAC affinity media (UniIDA/UniNTA), and other specific group affinity media (NM90 Agarose Blue, NW Rose GST 4FF).

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